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vector immpress duet immunohistochemistry kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vector immpress duet immunohistochemistry kit
    Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. <t>Immunohistochemistry</t> showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.
    Vector Immpress Duet Immunohistochemistry Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+immpress+duet+immunohistochemistry+kit/ImmPRESS+Duet+Double+Staining+HRP%2FAP+Polymer+Kit+(anti-rabbit+IgG-brown%2C+anti-mouse+IgG-red)/pmc10912671-181-16-23
    Average 94 stars, based on 47 article reviews
    vector immpress duet immunohistochemistry kit - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "A humanized mouse model for adeno-associated viral gene therapy"

    Article Title: A humanized mouse model for adeno-associated viral gene therapy

    Journal: Nature Communications

    doi: 10.1038/s41467-024-46017-0

    Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. Immunohistochemistry showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.
    Figure Legend Snippet: Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. Immunohistochemistry showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.

    Techniques Used: Injection, Expressing, Immunohistochemistry, Staining

    Related Articles

    Immunofluorescence:

    Article Title: A humanized mouse model for adeno-associated viral gene therapy
    Article Snippet: Co-staining of tdTomato (red for IF, brown for IHC) and hLDH (green for IF, magenta for IHC) required antigen retrieval with citrate buffer pH 6.0 for 30 min. After blocking with 2.5% horse serum, samples were incubated overnight at 4 °C with mouse monoclonal anti-hLDH antibody (Santa Cruz cat# LDH (H-10) sc-133123) in combination with rabbit anti-RFP antibody (Rockland, cat# 600–401–379), both diluted 1:100 in antibody diluent (Abcam, cat# ab642111) at 4 °C. .. Immunofluorescence (IF) was developed using fluorescent-labeled secondary antibodies (Jackson ImmunoResearch Laboratories) and IHC was developed following Vector ImmPRESS Duet immunohistochemistry kit manufacturer’s instructions (Vector Labs, cat# MP-7714–15). ..

    Immunohistochemistry:

    Article Title: A humanized mouse model for adeno-associated viral gene therapy
    Article Snippet: Co-staining of tdTomato (red for IF, brown for IHC) and hLDH (green for IF, magenta for IHC) required antigen retrieval with citrate buffer pH 6.0 for 30 min. After blocking with 2.5% horse serum, samples were incubated overnight at 4 °C with mouse monoclonal anti-hLDH antibody (Santa Cruz cat# LDH (H-10) sc-133123) in combination with rabbit anti-RFP antibody (Rockland, cat# 600–401–379), both diluted 1:100 in antibody diluent (Abcam, cat# ab642111) at 4 °C. .. Immunofluorescence (IF) was developed using fluorescent-labeled secondary antibodies (Jackson ImmunoResearch Laboratories) and IHC was developed following Vector ImmPRESS Duet immunohistochemistry kit manufacturer’s instructions (Vector Labs, cat# MP-7714–15). ..



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    vector immpress duet immunohistochemistry kit  (Vector Laboratories)
    94
    Vector Laboratories vector immpress duet immunohistochemistry kit
    Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. <t>Immunohistochemistry</t> showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.
    Vector Immpress Duet Immunohistochemistry Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+immpress+duet+immunohistochemistry+kit/ImmPRESS+Duet+Double+Staining+HRP%2FAP+Polymer+Kit+(anti-rabbit+IgG-brown%2C+anti-mouse+IgG-red)/pmc10912671-181-16-23
    Average 94 stars, based on 1 article reviews
    vector immpress duet immunohistochemistry kit - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. Immunohistochemistry showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.

    Journal: Nature Communications

    Article Title: A humanized mouse model for adeno-associated viral gene therapy

    doi: 10.1038/s41467-024-46017-0

    Figure Lengend Snippet: Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. Immunohistochemistry showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.

    Article Snippet: Immunofluorescence (IF) was developed using fluorescent-labeled secondary antibodies (Jackson ImmunoResearch Laboratories) and IHC was developed following Vector ImmPRESS Duet immunohistochemistry kit manufacturer’s instructions (Vector Labs, cat# MP-7714–15).

    Techniques: Injection, Expressing, Immunohistochemistry, Staining